Evaluation of Antitumor Activity of Crotalaria Burhia Buch.-Ham. Roots Against Ehrlich's Ascites Carcinoma Treated Mice

摘要

The study was aimed to evaluate antitumor activity of methanolic extract of Crotalaria burhia Buch.-Ham. roots (MECB) in Swiss Albino mice against Ehrlich's Ascites Carcinoma (EAC). The mice were divided into seven groups (n=20) in which Group I was treated with Sterile Physiological Saline (SPS) (Normal mice), Group II served as EAC control and Group III, IV, V or VI were treated with MECB at 100, 150, 300 or 400 mg/kg body weight i.p., respectively. Group VII were treated with standard drug 5-Fluorouracil (20 mg/kg i.p). The MECB was administered for 9 consecutive days. After 24 hours of last dose and 18 h of fasting, ten mice from each group were sacrificed and remaining mice from each group were kept to evaluate mean survival time. Antitumor activity of MECB was assessed by determining tumor volume, tumor weight, viable cells and nonviable cells count, hematological parameters, biochemical parameters and liver antioxidant status of EAC bearing host. The MECB was shown potent dose dependent antitumor activity. MECB at different doses level showed significant (P<0.05) reduction in tumor volume, tumor weight, and viable cells count with increased the life span of EAC mice. Interestingly, no mortality was observed during nine consecutive days of treatment. In further, the imbalanced hematological parameters of tumor bearing mice was significantly (P<0.05) regained after treatment of MECB. Moreover, altered biochemical parameters such as SGOT, SGPT, ALT, albumin and total protein in EAC mice were regained after treatment of MECB.