SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF PYRIDOXINE HYDROCHLORIDE AND DOXYLAMINE SUCCINATE IN TABLETS

摘要

A UV spectrophotometric method has been developed and validated for simultaneous estimation of pyridoxine hydrochloride and doxylamine succinate in tablet formulations using UV (Shimadzu) 1700 spectrophotometer with 0.1N HCI as solvent. The Amax of pyridoxine hydrochloride and doxylamine succinate was found to be 290 and 262 nm respectively in 0.1N HGI. Absorptivity of pyridoxine hydrochloride and doxylamine succinate at 290 and 262 nm was 460.64, 97.72 and 73.31, 249.82 respectively. The linearity for both pyridoxine hydrochloride and doxylamine succinate was in the range of 5-50 mcg/mL The mean recoveries obtained for pyridoxine hydrochloride and doxylamine succinate were 95.7-98.5% and 95.2-98.7% respectively. The developed method was found to be accurate, precise and rapid for the simultaneous estimation of pyridoxine hydrochloride and doxylamine succinate in tablets.