Influence of plant growth regulators and light on callus induction and bioactive phenolic compounds production in Pyrostegia venusta (Bignoniaceae)

摘要

Callus culture allows production of bioactive compounds in a short time when there is limited availability of natural sources. In this work, we analyzed the influence of plant growth regulators and light on callus induction and the phenolic compounds contents in the calli of Pyrostegia venusta. Leaf explants were placed on MS medium containing 2,4-dichlorophenoxiacetic acid and 6-benzylaminopurine in the presence and absence of light. Callus induction was observed in media with plant growth regulators. The calli's colour and consistency ranged from green and compact to yellow and friable, respectively in the presence and absence of light. The interaction between 4.52 mu M 2,4-D and 8.88 mu M BAP in the absence of light provided the best friable calli, with total phenol and flavonoid contents at 0.25 +/- 0.01 mu g ATE mg(-1) DW and 0.15 +/- 0.02 mu g RE mg(-1) DW, respectively. Calli presented higher flavonoid contents than those in the initial explants. HPLC-DAD analyses showed bioactive phenolic compounds as gallic, caffeic and benzoic acid derivatives in hydromethanolic extracts of calli.