A sensitive biosensor based on a ferrocene-marked adapter for the fluorescence detection of platelet-derived growth factor BB

摘要

A novel fluorescent nanometer biosensor integrating a beta-cyclodextrin-modified carbon dot (beta-CD-CD) fluorescent nanoprobe and ferrocene-marked adapter (ssDNA-Fc) was developed and used to measure platelet-derived growth factor (PDGF)-BB. When no PDGF-BB was added, ssDNA-Fc induced the enhanced fluorescence of beta-CD-CD nanoprobes. After adding PDGF-BB, ssDNA-Fc specifically bonded with PDGF-BB. Meanwhile, the beta-CD-CDs and Fc in ssDNA-Fc initiated host-guest identification, which then induced photoinduced electron transfer and decreased fluorescence, thereby contributing to the high-sensitivity monitoring of PDGF-BB. This novel approach showed a wide linear range of 10 pg mL 1 to 8 mu g mL(-1) and a low detection limit of 6 pg mL(-1) (RSD = +/-.4.6%). Moreover, the spiked recovery rate in human serum was 98.2%-106.2% (RSD <= +/- 3.79%), indicating that this method can be used to monitor PDGF-BB in human serum.