Antioxidant, cytotoxic and alpha-glucosidase inhibition activities from the Mexican berry 'Anacahuita' (Cordia boissieri)

摘要

This study describes the total phenolic and flavonoid content as well as cytotoxic, alpha-glucosidase inhibition and antiradical/antioxidant potential of extracts obtained from the edible fruits of Cordia boissieri, which is widely distributed throughout northeastern Mexico. Phenolic and flavonoid content were evaluated by means of the Folin-Ciocalteu method and aluminum chloride colorimetric assay respectively. The antiradical/antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and Trolox Equivalent Antioxidant Capacity (TEAC) assays. Cytotoxic activity was assessed by means of human cancer cell lines (MCF-7 and HeLa), alpha-glucosidase inhibition was determined by colorimetric assay using p-Nitrophenyl alpha-D-glucopyranoside (PNPG) as a substrate. Results indicate that extract of C. boissieri fruit has a good antioxidant potential to show a EC50 : 137.76 +/- 35 mu g/mL and 65 +/- 2 mu M/g in the DPPH and TEAC assays respectively, inhibitor of the enzyme alpha-glucosidase involved in sugar uptake (IC50 : 215.20 +/- 35 mu g/mL), cytotoxic activities against MCF-7 (IC50: 310 +/- 42 mu g/mL) and HeLa (IC50 : 450.4 +/- 21 mu g/mL) cancer cell lines as well as an important phenolic content with 230 +/- 23 mg/100g and 54 +/- 11 mg/100g of phenols and flavonoids totals respectively. These results point towards an interesting potential for the fruits of C. boissieri as chemopreventive properties and expand the possibilities for agro-industrial uses.