首页> 外文期刊>Acta Veterinaria Brno >Determination residues of penicillin G and cloxacillin in raw cow milk using Fourier transform near infrared spectroscopy.
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Determination residues of penicillin G and cloxacillin in raw cow milk using Fourier transform near infrared spectroscopy.

机译:使用傅立叶变换近红外光谱法测定原奶中青霉素G和氯西林的残留量。

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The study was conducted to determine the accuracy of Fourier transform near infrared spectroscopy (FTNIR) combined with the partial least square (PLS) method in determining the penicillin and cloxacillin residues in raw milk. The spectra were measured in the reflectance mode with transflectance cell in the spectral range of 10 000-4000/cm with 100 scans. Calibration models were developed, and were assessed statistically based on correlation coefficients (R) and standard errors of calibration (SEC). The following values were established for penicillin and cloxacillin R=0.951 and SEC=0.004, and R=0.871 and SEC=0.007, respectively. These calibration models were verified later with cross-validation. It was observed that better results were obtained in the calibration and validation models that were developed on milk samples coming from one farm. The maximum residue limit (MRL) of cloxacillin in milk could be determined by FT-NIR. However, standard errors of calibration and validation for penicillin G exceed the fixed MRL. It is concluded that FT-NIR spectroscopy is not a suitable method for accurate determination of penicillin and cloxacillin in raw milk. Differences in milk composition has a major influence on the detection of substances present at very low concentrations.
机译:该研究旨在确定傅里叶变换近红外光谱(FTNIR)结合偏最小二乘(PLS)方法测定原料奶中青霉素和氯西林残留的准确性。用透射反射池在100000-4000 / cm的光谱范围内以反射模式测量光谱,并进行100次扫描。开发了校准模型,并根据相关系数(R)和校准的标准误差(SEC)进行了统计评估。青霉素和氯西林的以下值分别建立为R = 0.951和SEC = 0.004,以及R = 0.871和SEC = 0.007。这些校准模型随后通过交叉验证进行了验证。据观察,在对来自一个农场的牛奶样品开发的校准和验证模型中,获得了更好的结果。牛奶中氯西林的最大残留限量(MRL)可以通过FT-NIR确定。但是,青霉素G的校准和验证标准误差超出了固定的MRL。结论是,FT-NIR光谱法不是准确测定生乳中青霉素和氯西林的合适方法。牛奶成分的差异对检测极低浓度物质的影响很大。

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