Immunomodulating effect of methisoprinol on the pronephros macrophage and lymphocyte activity after suppression induced by infectious haematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss)

摘要

The purpose of this in vitro study was to determine the influence of methisoprinol on the activity of pronephros macrophages and lymphocytes after suppression induced by infectious haematopoietic necrosis virus (IHNV). For this study, IHNV-free rainbow trout were used. Pronephros from 20 fish were removed, and single leukocyte suspensions were separated. The IHNV significantly (P<0.05) decreased the respiratory burst activity and potential killing activity of pronephros macrophages andproliferative response of lymphocytes stimulated by mitogens ConA and LPS. The results showed that methisoprinol at a concentration of 50 mg/ml modulated (restored) the metabolic and potential killing activity of macrophages and proliferative response of lymphocytes suppressed by IHNV.