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Gene expression of T lymphocytes of gravid cows during preimplantation

机译:妊娠前妊娠牛T淋巴细胞的基因表达

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摘要

An interaction between the conceptus and the immune system of animals is important during implantation. The aim of this study was to clarify the gene expression of T cell subsets in gravid cows during the preimplantation period. Peripheral blood from 14 Holstein dairy cows was. taken 14 days after artificial insemination. Based on the gravidity, cows were divided into gravid (n = 8) and nongravid (n 6) groups. Mononuclear cells from peripheral blood were stimulated with phytohaemagglutinin and then CD4(+), CD8(+), and WC1(+)gamma delta T cell subsets were isolated using magnetic cell sorting. The expression of interferon gamma, interleukin 4, and progesterone induced blocking factor were determined using real-time PCR. The expression of interleukin 4 and progesterone induced blocking factor was significantly higher in WC1(+)gamma delta T cells from gravid cows. In addition, interleukin 4 expression in WC1(+)gamma delta T cells from gravid cows was significantly higher than that in CD4(+) and CD8(+) T cells. This study describes for the first time the important role of WC1(+)gamma delta T cells during the preimplantation period in cows.
机译:在植入过程中,概念与动物免疫系统之间的相互作用非常重要。这项研究的目的是弄清楚妊娠前母牛在妊娠母牛中T细胞亚群的基因表达。来自14头荷斯坦奶牛的外周血。人工授精后14天服用。根据重度,将母牛分为重度(n = 8)和非重度(n 6)组。用植物血凝素刺激外周血中的单核细胞,然后使用磁性细胞分选法分离CD4(+),CD8(+)和WC1(+)γ-δT细胞亚群。干扰素γ,白介素4和孕激素诱导的阻断因子的表达使用实时PCR确定。妊娠牛的WC1(+)γ-δT细胞中白介素4和孕激素诱导的阻断因子的表达明显更高。此外,白羊的WC1(+)γ-δT细胞中白介素4的表达明显高于CD4(+)和CD8(+)T细胞。这项研究首次描述了WC1(+)γ-δT细胞在奶牛着床前期的重要作用。

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