Paraburkholderia panacisolisp. nov., a potentially antagonistic bacterium against the root rot fungal pathogenCylindrocarpon destructans, isolated from ginseng cultivation soil

摘要

A new bacterium, designated DCY113(T), was isolated from ginseng cultivation soil in Gochang-gun, South Korea, and its taxonomic position identified by the polyphasic approach. 16S rRNA gene sequence analysis determined that this isolate belongs to the genusParaburkholderia,and was closest toP. dipogonisDL7(T)(98.6%),P. phytofirmansPsJN(T)(98.5%),P. kirstenboschensisKb15(T)(98.4%) andP. aromaticivoransBN(T)(98.1%). Strain DCY113(T)is Gram-reaction negative, strictly aerobic, rod-shaped, non-motile, and catalase and oxidase positive. The predominant isoprenoid quinone of DCY113(T)was ubiquinone Q-8. The major cellular fatty acids were C-16:0, cyclo-C(17:0)and the Summed feature 8 (C-18:1 omega 7cand/or C-18:1 omega 6c). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and an unknown amino lipid (AL1). The G+C content of the genomic DNA was 62.2 mol%. Average nucleotide identity (ANI) between strain DCY113(T)and the relatedParaburkholderiatype strains were below the threshold value for species delineation. This low DNA relatedness in combination with phylogenetic and phenotypic tests indicates that strain DCY113(T)cannot be assigned to any recognized species. Strain DCY113(T)was also found to have antifungal activity against the pathogenic fungiCylindrocarpon destructans. In conclusion, this study found DCY113(T)to be a novel species within the genusParaburkholderia, for which the nameP. panacisoliis proposed. The type strain is DCY113(T)(= KCTC 52951(T) = JCM 32098(T)).