首页> 外文期刊>Acta Veterinaria Brno >Protein and antigenic profile among Mycoplasma bovis field strains isolated in Bosnia and Herzegovina.
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Protein and antigenic profile among Mycoplasma bovis field strains isolated in Bosnia and Herzegovina.

机译:波斯尼亚和黑塞哥维那分离的牛支原体田间菌株的蛋白质和抗原谱。

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摘要

Mycoplasma bovis is a pathogenic disease distributed worldwide, but is often overlooked. This pathogen causes respiratory disease, mastitis, and arthritis in cattle. This study was conducted to characterize the protein and antigenic profiles of M. bovis field strains isolated from Bosnia and Herzegovina using sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting, and to analyse the possible variations among these strains. Results revealed that significant differences occurred when field strains were compared with the reference strain PG45. One field strain isolated from lung samples of a heifer was significantly different from strains isolated from nasal swabs taken from cattle raised in another geographic region. It is suggested that a possible correlation exists between protein and antigen profiles of M. bovis field strains, geographic regions and anatomical sites of isolation.
机译:牛支原体是一种分布在世界各地的致病性疾病,但经常被忽视。该病原体引起牛的呼吸系统疾病,乳腺炎和关节炎。进行该研究以表征 M的蛋白质和抗原谱。使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳和免疫印迹法从波斯尼亚和黑塞哥维那分离的牛原野菌株,并分析了这些菌株之间可能的变异。结果表明,将现场菌株与参考菌株PG45进行比较时,发生了显着差异。从小母牛的肺部样本中分离出的一种田间菌株与从另一地理区域饲养的牛中采集的鼻拭子中分离出的菌株显着不同。提示在蛋白质和iM的抗原谱之间存在可能的相关性。牛的野外应变,地理区域和孤立的解剖部位。

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