MOLECULAR TAXONOMIC ANALYSIS OF MONOTROPA HYPOPITYS AND ITS RELATIONSHIP WITH PYROLACEAE

摘要

The relations between Monotropa hypopitys andfour members of Pyrolaceae collected in Bulgaria were investigated using five ISSR markers. The genetic differences given by each usedprimer were illustrated graphically. The selective ability of each primer was discussed according to the obtained results. The phylogenetic relationships were illustrated using a resulting dendrogram constructed with the data from all polymorphic variants. Monotropa was separated in a single clade only by one marker ana displays highest genetic similarity with Orthilia secunda. Our results suggested that Monotropoideae may belong to Pyrolaceae, together with Pyroloideae. Both groups represent two mycoheterotropic lines dependent on the nutrition, which determines their distribution. Monotropa is the terminal result of the gradual passage from authotrophy to mycorrhizal parasitism. In spite of the fact that Monotropaceae is known as a paraphyletic family, it showed a close similarity to Orthilia. The data supported the presumption that Monotropa hypopitys has to be included in the boundaries of Pyrolaceae. The obtained results could serve as a starting point to reconsider the existing classification based on morphological observations.