首页> 外文期刊>Biotechnology & Biotechnological Equipment >PCR-SSCP-DNA SEQUENCING METHOD IN DETECTING PTEN GENE MUTATION AND ITS SIGNIFICANCE IN HUMAN BREAST CANCER IN TURKISH POPULATIONS
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PCR-SSCP-DNA SEQUENCING METHOD IN DETECTING PTEN GENE MUTATION AND ITS SIGNIFICANCE IN HUMAN BREAST CANCER IN TURKISH POPULATIONS

机译:PCR-SSCP-DNA测序技术在土耳其人群乳腺癌中检测PTEN基因突变及其意义

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The aim of this study was to analyze the possible effect of PTEN gene mutations on the occurrence and development of human breast cancer. PCR-SSCP-DNA sequencing indicated that two kinds of mutation sites were found in 4 out of 80 breast cancer samples. One kind of mutation was found in exons. AA-TCC mutation was located 40 bp upstream of the 3’ lateral exon 2 (115946 AA-TCC). Such mutations led to terminator formation at codon 267 of the PTEN gene. The other mutation was found in an intron, including a C-T point mutation 91 bp upstream of 2’ lateral exon 2 (1903858 C-T). The PTEN protein expression in tissues with breast cancer indicated that the total positive rate of PTEN protein expression was 68 % in breast cancer tissue, which was significantly lower than that (100 %) in paracancerous tissues (P < 0.005). PTEN gene mutation may play an important role in the occurrence and development of breast cancer.
机译:这项研究的目的是分析PTEN基因突变对人类乳腺癌发生和发展的可能影响。 PCR-SSCP-DNA测序表明,在80个乳腺癌样本中有4个发现了两种突变位点。在外显子中发现了一种突变。 AA-TCC突变位于3'侧向外显子2(115946 AA-TCC)上游40 bp。此类突变导致在PTEN基因的第267位密码子处形成终止子。在内含子中发现了另一个突变,包括在2'侧外显子2(1903858 C-T)上游91 bp处的C-T点突变。乳腺癌组织中PTEN蛋白的表达表明,乳腺癌组织中PTEN蛋白表达的总阳性率为68%,显着低于癌旁组织中的PTEN蛋白表达(100%)(P <0.005)。 PTEN基因突变可能在乳腺癌的发生和发展中起重要作用。

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