FORMATION OF PSEUDORABIES VIRUS GLYCOPROTEIN E/I COMPLEX IN BACULOVIRUS RECOMBINANT SYSTEM

摘要

Glycoproteins E (gE) and 1 (gl) of Pseudorabies virus (PRV) form a non-covalently bound complex to which a number of functions have been attributed. The gE/gl complex formation was studied using a series of full-length and truncated forms of gE and gl expressed in baculovirus recombinant system. Both glycoproteins were truncated by stepwise removal of their C-terminal parts and their ability to form the complex was studied by radioimmunoprecipitation. It was found that N-terminal domains of gE and gl containing first 122 and 106 aa, respectively, were sufficient for the complex formation.