Preparation of recombinant coat protein of Prunus necrotic ringspot virus

摘要

The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET16b vector and expressed in Escherichia coil. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x E for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).