Biotransformation of Glycoginsenosides to Intermediate Products and Aglycones using a Hemicellulosome Produced by Cellulosimicrobium cellulan

摘要

Ginsenosides Rb1, Rb2 and Re were converted into corresponding intermediates and aglycones using a multienzyme complex produced by Cellulosimicrobium cellulans F16. The whole conversion processes were monitored at different time points by UPLC-PDA-MS. The hydrolysates and the conversion routes were identified using standard samples and electrospray ionization MS. Ginsenosides Rb1, Rb2, and Re were finally converted to the product protopanaxadiol and ginsenoside Rg2. The results indicated that the multienzyme complex Xyl I produced by C. cellulans F16 can be an efficient tool for the transformation of ginsenosides.