Partial molecular characterization of an unusual, recombinant Plum pox virus isolate from Bulgaria.

摘要

Plum pox virus (PPV; the genus Potyvirus, the family Po-tyviridae), the causal agent of sharka disease, has been classified into six groups of isolates PPV-M, PPV-D, PPV-Rec, PPV-EA, PPV-C, and PPV-W according to their serological and molecular features (1-3). The PPV-Rec group contains viral isolates that emerged from a natural recombination between PPV-D and PPV-M isolates with a recombination breakpoint located in the NIib gene. Later on another recombination point was detected in P3 gene (2).It has been demonstrated that there is a strong correlation among the different techniques used to classify the isolates belonging to PPV-M and PPV-D groups (1, 4-8). However, the serological and molecular typing of some PPV isolates gave inconsistent results, when CP protein or gene was examined (1, 9).