Electron microscopic observation of potato virus a using murine monoclonal antibodies.

摘要

Six monoclonal antibodies (MoAbs) against potato A potyvirus (PVA) were prepared and used in ELISA, immunoblot analysis and electron microscopic studies of the virus. Four MoAbs, 151, 290, 328 and 634, reacted with purified virus preparation in dotblot tests and also showed a strong reaction with virus coat protein (CP) denatured by sodium dodecyl sulfate (SDS), while 2 MoAbs, 534 and 187, gave a significantly weaker reaction with denatured CP than with purified virus. On electron micrographs, MoAb 534 effected binding only on a few separate locations of the virus surface after prolonged storage. It is presumed that this MoAb recognized a conformation-dependent epitope.