High-throughput detection of DNA double-strand breaks using image cytometry

摘要

Assessment of gamma H2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated image cytometry assessment of cell average gamma H2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for gamma H2AX analysis.