Flavisolibacter carri sp nov., isolated from an automotive air-conditioning system

摘要

A Gram-stain negative, aerobic, non-motile, rod-shaped and yellow bacterium, designated TX0651(T), was isolated from an automotive air-conditioning system. Phylogenetically, the strain groups with the members of the genus Flavisolibacter and exhibits high 16S rRNA gene sequence similarities with Flavisolibacter ginsenosidimutans Gsoil 636(T) (97.4%), Flavisolibacter ginsengiterrae Gsoil 492(T) (96.3%) and Flavisolibacter ginsengisoli Gsoil 643(T) (96.2%). DNA-DNA relatedness between TX0651(T) and F. ginsenosidimutans KCTC 22818(T) and F. ginsengiterrae KCTC 12656(T) were determined to be less than 40%. The low levels of DNA-DNA relatedness identifies the strain TX0651(T) as a novel species in the genus Flavisolibacter. The major cellular fatty acids were identified as iso-C-15:0, summed feature 3 (C(16:1)7c and/or C(16:1)6c), iso-C-15:1 G and iso-C-17:0 3-OH. The predominant respiratory quinone was identified as MK-7. The polar lipids were found to be comprised of phosphatidylethanolamine, unidentified amino-glycophospholipids, an unidentified aminophospholipid, an unidentified amino lipid and unidentified lipids. The DNA G+C content of the strain was determined to be 31.2mol%. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain TX0651(T) should be classified in a novel species in the genus Flavisolibacter, for which the name Flavisolibacter carri sp. nov. (=KACC 19014(T)=KCTC 52836(T)=NBRC 111784(T)) is proposed.