In vitro evaluation of nebivolol effects on nonadrenergic noncholinergic responses in rabbit corpus cavernosum

摘要

Abstract The purpose of this study was to compare the effects of nebivolol on nonadrenergic noncholinergic ( NANC ) relaxation functions that are mediated by electric field stimulation ( EFS ) in rabbit corpus cavernosum smooth muscle by comparison with other beta‐adrenergic receptor blockers and show the level on which its effects through nitric oxide take place. After the effects of nebivolol on the isolated corpus cavernosum tissues that were contracted through the alpha‐adrenergic pathway and application of L‐ NAME ’ ( N G ‐nitro‐L‐arginine methyl ester) which is a competitive inhibitor of nitric oxide synthase ( NOS ), the changes that occurred were recorded. Following the effect on the tissue that was contracted with phenylephrine in the presence of atropine and guanethidine that was created by EFS , nebivolol and other beta‐blockers were added and the changes were recorded. After receiving relaxation responses with EFS ‐mediated NANC , no difference was observed between the relaxation responses due to addition of nebivolol and other beta‐adrenergic blockers ( p ??0.05). The finding that nebivolol which has a NO ‐mediated relaxation effect did not have an effect on EFS ‐mediated NANC relaxation but created relaxation on the tissue that was contracted by phenylephrine and the effect was reversed by L‐ NAME , shows that its effects are on a postsynaptic level.