A fast-responding, highly sensitive detection system consisting of a fluorescent probe and palladium ions for N2H4 in environmental water and living cells

摘要

Hydrazine (N2H4), as a chemical material and high-energy fuel, has been widely used in industry and aerospace. However, the large-scale application of N2H4 poses a great risk to the environment and humans due to the strong corrosiveness and toxicity of N2H4. Herein, based on the reaction mechanisms of N2H4-induced Pd2+ reduction, Pd-0-mediated allyl cleavage and intramolecular cyclization, a new fluorescent system consisting of a probe BINC and Pd2+ was designed for detecting N2H4. After adding N2H4, the BINC-Pd2+ system emits bright green fluorescence in less than 10 minutes, which is suitable for rapid naked-eye recognition of N2H4. Even when coexisting with various cations, anions, biological substances and amines, the BINC-Pd2+ system exhibits a selective and stable fluorescence response to N2H4. Moreover, the fluorescence intensity at 519 nm has good linearity with the N2H4 concentration in the range of 0-10 mu M. The detection limit is 26 nM (0.83 ppb), which is lower than the threshold limit (10 ppb) of N2H4 in drinking water. Our results indicate that the BINC-Pd2+ system can be used for quantitative analysis of N2H4 in environmental water with recovery rates of 95.1-103.9%, as well as fluorescence imaging of N2H4 in living cells.