Analysis of Phlebodium decumanum Fronds by High-Performance Liquid Chromatography by Ultraviolet-Visible and Quadrupole Time-of-Flight Tandem Mass Spectrometry (HPLC-UV-VIS-QTOF-MS/MS)

摘要

Phlebodium decumanum (P. decumanum), together with other tropical ferns commonly known as Calaguala, have been empirically applied since ancient times to ameliorate inflammatory disorders, skins diseases and even cancer. There are evidences of antineoplastic potential and anti-inflammatory, immunomodulatory and antioxidant properties of the extract. Preliminary studies have also shown direct antitumor activity of the extracts. In the present article, the phytochemical composition of a hydro-ethanolic extract of P. decumanum is investigated using high-performance liquid chromatography (HPLC) with ultraviolet-visible (UV-vis) diode array detection (DAD) and electrospray ionization (ESI) quadrupole time-of-flight tandem mass spectrometry (QTOF-MS/MS) detection. First, the chromatographic profile is established as a representative fingerprint of the compounds extracted from the fern. Then, a total of 122 chemicals, including 23 flavonoids, 47 phenolic acids (34 hydroxycinnamic acids and 13 hydroxybenzoic acids), 9 amino and amino-sugar derivatives, 24 organic acids and their derivatives, and other metabolites, have been characterized. Moreover, 12 unknown compounds were also detected, providing the first comprehensive characterization available on the phytochemical composition of the leaves of P. decumanum.