Air Assisted Dispersive Liquid-Liquid Microextraction (AA-DLLME) Using Hydrophilic-Hydrophobic Deep Eutectic Solvents for the Isolation of Monosaccharides and Amino Acids from Kelp

摘要

Air assisted dispersive liquid-liquid microextraction (AA-DLLME) using hydrophilic-hydrophobic deep eutectic solvents (DES) was developed for the simultaneous isolation of monosaccharides and amino acids with wide ranges of polarities from kelp using high performance liquid chromatography (HPLC). A response surface methodology (RSM) on a Box-Behnken design (BBD) model was employed to identify the optimal extraction parameters. Air assisted dispersive liquid-phase microextraction performed using the optimum deep eutectic solvent system, five push-pull cycles, a ratio of solid to liquid equal to 3mg·mL~(-1), 10% (w/v) NaCl, and a centrifugation time of 6 min provided the best analytical performance. The optimal extracted concentrations of D-(+)-galactose, L-(-)-fucose, DL-tyrosine, and DL-valine in kelp were 16.7 ± 0.2, 8.6 ± 0.2, 2.6 ± 0.1, and 1.6 ± 0.1mg·g~(-1), respectively. The method recoveries for D-(+)-galactose, L-(-)-fucose, DL-tyrosine, and DL-tyrosine were from 87 to 102%, 84 to 103%, 87 to 104%, and 85 to 103%. The relative standard deviations (RSDs) (n=4) for the intra-day and inter-day determinations were <6.17%.