A RP-HPLC Method for Estimation of Tramadol HCl and Chlorzoxazone in Tablet Formulation-Validation

摘要

A simple, rapid and selective method involving reverse phase liquid chromatography is developed for the concurrent determination of Tramadol HCl and Chlorzoxazone in tablet formulation. Chromatographic separation of the two drugs was performed on Promosil C18 column (250 nun x 4.6 mm, 5 μm) as stationary phase with a mobile phase comprising of Acetonitrile and 0.1% Trifluroaceticacid in water in the ratio 60:40 (v/v) at a flow rate of 1.0 mL/min and UV detection at 275 nm. The linearity of Tramadol HCl and Chlorzoxazone were in the range of 25.00 μg/mL-45.00 μg/mL and 125.00 μg/mL-225.00 ug/mL respectively. Standard addition method was used to calculate percentage recovery. The average amount of recovery was found to be 98.8% and 99.7% for Tramadol HCl and Chlorzoxazone respectively. The proposed method was found to be accurate, precise and rapid for the simultaneous determination of Tramadol HCl and Chlorzoxazone.