Perfusion Medium Development for Continuous Bioprocessing of Animal Cell Cultures

摘要

Traditional bioprocesses of mammalian cell cultures are operated in batch- or fed-batch mode. To increase space-time-yields, reduce cost of goods, accelerate time-to-market and enhance flexibility at reduced lab-footprint, perfusion processes are envisaged for various bioprocess intensification tasks. The goal of dynamic perfusion is to achieve high viable cell densities (VCD) by maintaining high specific growth rates over prolonged time-periods. It is usually operated at high perfusion rates to generate the required biomass for the establishment of high-density cryopreserved cell banks, or as inoculum in the N-1 stage for larger production bioreactors operated as (intensified) fed-batch or concentrated fed-batch cultures. Dynamic perfusion operation can be switched to a continuous, steady-state perfusion culture at low perfusion rates and constantly high VCD over several weeks or even months for efficient product generation at high quality. Depending on the specific goal of perfusion operation a suitable medium should support maximum growth or productivity.