RNA Extraction from Gastrointestinal Tract and Pancreas by a Modified Chomczynski and Sacchi Method

摘要

The development of effective and reproducible RNA isolation techniques depends on inhibition of contaminating RNases, enrichment of mRNA species and elimination of contaminating DNA. Moreover, it should be possible to process many samples simultaneously. Guanidinium thiocyanate (GTC) and guanidinium chloride are effective and commonly used protein denatur-ants. Chomczynski and Sacchi described a modified GTC procedure that relies on extraction of the GTC-lysate with phenol-chloroform at low pH.