Optimized insect cell culture for the production of recombinant heterologous proteins and Baculovirus particles

摘要

Insect cells are natural hosts for baculoviruses. Following infection, nuclear polyhedral inclusion bodies (PIBs) and budded virus (BV) are produced. Virion-rich PIBs are ingested by insect larvae as food contaminants. Upon PIB digestion in the insect midgut, virions replicate in midgut cells and spread to other tissues, leading larvae to become a fluid-filled sac containing large amounts of new PIBs. PIBs derived from the Anticarsia gemmatalis multicapsid nuclear polyhedrosis virus (AgMNPV) have already been applied as a biopesticide against the soybean worm Anticarsia gemmatalis, and cell culture-derived PIB production on an industrial scale can be envisaged (1,4,15).