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A yeast surface display platform for plant hormone receptors: Toward directed evolution of new biosensors

机译：植物激素受体的酵母表面显示平台：朝向新生物传感器的指导演变

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Abstract > Small‐molecule biosensors have major applications in biotechnology and medicine but remain difficult to engineer. Plant hormone receptors represent an attractive platform for engineering such biosensors because their chemically induced dimerization architectures naturally decouple small‐molecule sensing and sensor actuation. Rapid biosensor engineering will require quantitative high‐throughput screening methods. Here we develop a yeast surface display (YSD) platform for the PYR1/HAB1 abscisic acid sensor of Arabidopsis thaliana . We extensively optimized PYR1 surface display, HAB1 purification, and binding reaction conditions. Our system reproduces previous results with wild‐type and engineered receptors, and a mathematical analysis of the PYR1/HAB1 system allows us to infer all binding constants. Critically, we find that a previously engineered PYR1 receptor with altered ligand specificity binds HAB1 with identical affinity, suggesting that substantial reengineering of the PYR1 binding pocket does not compromise sensor actuation. This YSD platform for A. thaliana PYR1/HAB1 will facilitate future biosensor engineering efforts. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 小分子生物传感器在生物技术和医学中具有主要应用，但仍然难以工程。植物激素受体代表了一种有吸引力的工程平台，因为它们的化学诱导的二聚化架构自然地解除了小分子感测和传感器致动。快速生物传感器工程将需要定量高通量筛选方法。在这里，我们为Pyr1 / Hab1脱落酸传感器开发了一种酵母表面显示器（YSD）平台 rapidopsis thaliana </ i> 。我们广泛优化Pyr1表面显示器，HAB1纯化和结合反应条件。我们的系统通过野生型和工程受体再现先前的结果，Pyr1 / Hab1系统的数学分析使我们能够推断所有结合常数。批判性地，我们发现具有改变的配体特异性的先前设计的Pyr1受体结合具有相同亲和力的HAB1，表明Pyr1结合口袋的基本再造不会损害传感器致动。这个ysd平台 a。蒂利亚纳</ i> Pyr1 / Hab1将促进未来的生物传感器工程努力。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-18537/'>《AIChE Journal》</a> <b style="margin: 0 2px;">|</b><span>2020年第3期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Steiner Paul J.&option=202" target="_blank" rel="nofollow">Steiner Paul J.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bedewitz Matthew A.&option=202" target="_blank" rel="nofollow">Bedewitz Matthew A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Medina‐Cucurella Angélica V.&option=202" target="_blank" rel="nofollow">Medina‐Cucurella Angélica V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cutler Sean R.&option=202" target="_blank" rel="nofollow">Cutler Sean R.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Whitehead Timothy A.&option=202" target="_blank" rel="nofollow">Whitehead Timothy A.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemical and Biological EngineeringUniversity of ColoradoBoulder Colorado;</p> <p>Department of Chemical and Biological EngineeringUniversity of ColoradoBoulder Colorado;</p> <p>Department of Chemical Engineering and Materials ScienceMichigan State UniversityEast Lansing Michigan;</p> <p>Department of Botany and Plant SciencesUniversity of CaliforniaRiverside California;</p> <p>Department of Chemical and Biological EngineeringUniversity of ColoradoBoulder Colorado;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/238.html" title="化学工业">化学工业;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biosensors&option=203" rel="nofollow">biosensors;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chemical induced dimerization&option=203" rel="nofollow">chemical induced dimerization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hormones&option=203" rel="nofollow">hormones;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=plant biotechnology&option=203" rel="nofollow">plant biotechnology;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=yeast surface display&option=203" rel="nofollow">yeast surface display;</a> </p> <div class="translation"> 机译：生物传感器;化学诱导的二聚化;激素;植物生物技术;酵母表面显示; </div> </li> <li> <span class="lefttit">入库时间</span> <span>2022-08-20 00:45:30</span> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022505722.html">A yeast surface display platform for plant hormone receptors: Toward directed evolution of new biosensors</a> 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