A Pre-Loading Method of Evaluating Gap Junctional Communication by Fluorescent Dye Transfer

摘要

We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and Dil Calcein is able to pass through gap junctions, while Dil is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this systemto evaluate dye transfer within a given period of time.