Producing Covalent Microarrays of Amine‐Conjugated DNA Probes on Various Functional Surfaces to Create Stable and Reliable Biosensors

Yousefi Hanie; Su Hsuan‐Ming; Ali Monsur; Filipe Carlos D. M.; Didar Tohid F.

摘要

Abstract > Oligonucleotide‐based microarrays are ideal tools for genetic testing and diagnostics due to their stability, ease of synthesis, and high specificity for the target of interest. This study reports on the effectiveness of several coupling strategies to covalently attach single‐stranded nucleic acids to surfaces, focusing on the robustness of the attachment in various environmental conditions, such as pH and temperature. Various characteristics of DNA microarrays produced using amine‐conjugated DNA probes on five different functional surfaces, commonly used for immobilization of biomolecules, namely, epoxy, carboxyl, amine, aldehyde, and N ‐hydroxysuccinimide‐coated substrates, are investigated. Immobilization efficiency, changes in surface energy, as well as the stability of the conjugated DNA upon exposure to various environmental conditions are measured. Finally, in order to study the postimmobilization viability of the developed biosensors, microarrays of synthetic RNA cleaving probes (DNAzyme) are immobilized onto these surfaces and their functionality is evaluated through their ability to detect Escherichia coli at various temperatures. Results show that epoxy‐coated plastic surfaces are most ideal for the creation of DNA‐based biosensing chips. This study provides a guideline for producing oligonucleotide‐based microarrays on glass and plastic substrates and can be used for developing microarray‐based biosensors, suitable for long‐term storage. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 基于寡核苷酸的微阵列是遗传检测和诊断的理想工具，由于它们的稳定性，易于合成，以及感兴趣的目标的高特异性。该研究报告了几种偶联策略的有效性，将单链核酸共价连接到表面，聚焦在各种环境条件下附着的鲁棒性，例如pH和温度。使用胺缀合的DNA探针在五种不同功能表面上产生的DNA微阵列的各种特征，通常用于固定生物分子，即环氧树脂，羧基，胺，醛和 n </ i> 研究 - 亚昔琥珀酰亚胺涂覆的基材。测量固定效率，表面能的变化，以及在暴露于各种环境条件时缀合DNA的稳定性。最后，为了研究开发的生物传感器的后化活力，将合成RNA切割探针（DNAzyme）的微阵列固定在这些表面上，并通过其检测能力来评估它们的功能大肠杆菌</ i> 在各种温度下。结果表明，环氧涂层的塑料表面是创建基于DNA的生物传感芯片的理想选择。该研究提供了在玻璃和塑料基材上生产基于寡核苷酸的微阵列的指导，并且可用于开发基于微阵列的生物传感器，适用于长期储存。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-18063/'>《Advanced materials interfaces》</a> <b style="margin: 0 2px;">|</b><span>2018年第16期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yousefi Hanie&option=202" target="_blank" rel="nofollow">Yousefi Hanie;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Su Hsuan‐Ming&option=202" target="_blank" rel="nofollow">Su Hsuan‐Ming;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ali Monsur&option=202" target="_blank" rel="nofollow">Ali Monsur;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Filipe Carlos D. M.&option=202" target="_blank" rel="nofollow">Filipe Carlos D. M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Didar Tohid F.&option=202" target="_blank" rel="nofollow">Didar Tohid F.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Mechanical EngineeringMcMaster University1280 Main Street West L8S 4L8 Hamilton Ontario Canada;</p> <p>Department of Mechanical EngineeringMcMaster University1280 Main Street West L8S 4L8 Hamilton Ontario Canada;</p> <p>Biointerfaces InstituteMcMaster University1280 Main Street West L8S 4L8 Hamilton Ontario Canada;</p> <p>Department of Chemical EngineeringMcMaster University1280 Main Street West L8S 4L8 Hamilton Ontario Canada;</p> <p>Department of Mechanical EngineeringMcMaster University1280 Main Street West L8S 4L8 Hamilton Ontario Canada;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/6960.html" title="特种结构材料">特种结构材料;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biosensors&option=203" rel="nofollow">biosensors;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=DNAzyme microarrays&option=203" rel="nofollow">DNAzyme microarrays;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=inkjet printing&option=203" rel="nofollow">inkjet printing;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=oligonucleotide microarrays&option=203" rel="nofollow">oligonucleotide microarrays;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=stable conjugation&option=203" rel="nofollow">stable conjugation;</a> </p> <div class="translation"> 机译：生物传感器;DNazyme微阵列;喷墨印刷;寡核苷酸微阵列;稳定的共轭; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022433729.html">Producing Covalent Microarrays of Amine‐Conjugated DNA Probes on Various 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