CYTOLOGICAL ASSESSMENT OF CARROT PLANTS OBTAINED IN ANTHER CULTURE

摘要

Anthers of Feria F-1 and Narbonne F-1 carrot cultivars were cultured in vitro to induce androgenic embryos. To confirm the microspore origin of developed embryos. chromosome counts of root Lip meristematic cells were made for each carrot plant obtained in anther culture. Using phase contrast technique and fluorescence microscopy. cytological changes of microspores during culture leading to proembryo formation were documented in the first days after anther placement on the induction medium. More than 90% of the carrot plants obtained in anther cultures had no haploid chromosomes.