Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro

摘要

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa ev. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, LAA, NAA, BAP, kinetin, 2iP) for regeneration. There were significant differences in regeneration response between medium supplemented with TDZ and medium with other hormones. Only medium containing TDZ stimulated shoot induction. The highest efficiency of shoot regeneration (avg. 6.2 shoots per culture) was on medium supplemented with 0.5 mg/l TDZ. The ploidy of callus and organs formed in endosperm culture was examined by flow cytometry. The results, peaks corresponding to 3C DNA amounts, confirmed the endospermal origin of callus, roots and shoots. Aneuploid and polyploid cells were found in endosperm-derived callus and regenerated organs.