Detection of circulating dendritic cells subsets by flow cytometry in peripheral blood of healthy subjects: Two different approaches

摘要

Background: Dendritic cells (DCs) are antigen presenting cells that play a crucial role in antitumor immune response. DCs can be detected in peripheral blood (PB) and divided into 2 subsets identified according to their function: myeloid DCs (DC1) and plasmocytoid DCs (DC2). As preliminary phase of a clinical study on cancer patients, we wanted to define DC and DC subset values in healthy subjects (HS). Methods: Samples of PB from 50 HS (M/F: 25/25, median age 40 yrs) were analysed.