Automated analysis of individual sperm cells using stain‐free interferometric phase microscopy and machine learning

机译：使用无染色干涉相位显微镜和机器学习自动分析单个精子细胞

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Abstract >Currently, the delicate process of selecting sperm cells to be used for in vitro fertilization (IVF) is still based on the subjective, qualitative analysis of experienced clinicians using non‐quantitative optical microscopy techniques. In this work, a method was developed for the automated analysis of sperm cells based on the quantitative phase maps acquired through use of interferometric phase microscopy (IPM). Over 1,400 human sperm cells from 8 donors were imaged using IPM, and an algorithm was designed to digitally isolate sperm cell heads from the quantitative phase maps while taking into consideration both the cell 3D morphology and contents, as well as acquire features describing sperm head morphology. A subset of these features was used to train a support vector machine (SVM) classifier to automatically classify sperm of good and bad morphology. The SVM achieves an area under the receiver operating characteristic curve of 88.59% and an area under the precision‐recall curve of 88.67%, as well as precisions of 90% or higher. We believe that our automatic analysis can become the basis for objective and automatic sperm cell selection in IVF. ? 2017 International Society for Advancement of Cytometry </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”> <title type =“main”>抽象</ title> >当前，选择精子单元的微妙过程用于体外施肥（IVF）仍然基于使用非定量光学显微镜技术的经验丰富的临床医生的主观性定性分析。在这项工作中，基于通过使用干涉性相位显微镜（IPM）获得的定量相位图来开发一种用于自动分析精子细胞的方法。使用IPM对来自8个供体的1,400多种人体精子细胞进行了成像，并且设计了一种算法，以在考虑细胞3D形态和内容的同时从定量相位映射数字分离精子细胞头，以及描述精子头形态的获取特征。这些功能的子集用于训练支持向量机（SVM）分类器以自动对精子进行良好和不良形态的精子。 SVM在接收器下的接收器的一个区域实现88.59％，并且在精密召回曲线下的区域为88.67％，以及90％或更高的精度。我们认为，我们的自动分析可以成为IVF中客观和自动精子细胞选择的基础。还2017年国际促进细胞术社会</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-17718/'>《Cytometry, Part A: the journal of the International Society for Analytical Cytology》</a> <b style="margin: 0 2px;">|</b><span>2017年第9期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mirsky Simcha K.&option=202" target="_blank" rel="nofollow">Mirsky Simcha K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Barnea Itay&option=202" target="_blank" rel="nofollow">Barnea Itay;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Levi Mattan&option=202" target="_blank" rel="nofollow">Levi Mattan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Greenspan Hayit&option=202" target="_blank" rel="nofollow">Greenspan Hayit;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shaked Natan T.&option=202" target="_blank" rel="nofollow">Shaked Natan T.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Biomedical EngineeringFaculty of Engineering Tel Aviv UniversityTel Aviv 69978 Israel;</p> <p>Department of Biomedical EngineeringFaculty of Engineering Tel Aviv UniversityTel Aviv 69978 Israel;</p> <p>Department of Cell and Developmental BiologySackler Faculty of Medicine Tel Aviv UniversityTel Aviv Israel;</p> <p>Department of Biomedical EngineeringFaculty of Engineering Tel Aviv UniversityTel Aviv 69978 Israel;</p> <p>Department of Biomedical EngineeringFaculty of Engineering Tel Aviv UniversityTel Aviv 69978 Israel;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/174.html" title="细胞生物学">细胞生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=in vitro fertilization&option=203" rel="nofollow">in vitro fertilization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=machine learning&option=203" rel="nofollow">machine learning;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=interference microscopy&option=203" rel="nofollow">interference microscopy;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=spermatozoa&option=203" rel="nofollow">spermatozoa;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=support vector machine&option=203" rel="nofollow">support vector machine;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=holography&option=203" rel="nofollow">holography;</a> </p> <div class="translation"> 机译：体外施肥;机器学习;干扰显微镜;精子;支持向量机;全息; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022396893.html">Automated analysis of individual sperm cells using stain‐free interferometric phase microscopy and machine learning</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mirsky Simcha K.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mirsky Simcha K.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Barnea Itay&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Barnea Itay,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Levi Mattan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Levi Mattan,</a> <a href="/journal-foreign-17718/" target="_blank" rel="nofollow" class="tuijian_authcolor">Cytometry, Part A: the journal of the International Society for Analytical Cytology .</a> <span>2017</span><span>,第9期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用无染色干涉相位显微镜和机器学习自动分析单个精子细胞</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704020419626.html">Re: Interferometric Phase Microscopy for Label-Free Morphological Evaluation of Sperm Cells</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Niederberger Craig&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Niederberger Craig </a> <a href="/journal-foreign-31006/" target="_blank" rel="nofollow" class="tuijian_authcolor">The Journal of Urology .</a> <span>2016</span><span>,第2期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：Re：干涉阶段显微镜，用于无标菌的精子细胞的形态学评价</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704019242952.html">Re: Interferometric Phase Microscopy for Label-Free Morphological Evaluation of Sperm Cells</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Niederberger Craig&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Niederberger Craig </a> <a href="/journal-foreign-31006/" target="_blank" rel="nofollow" class="tuijian_authcolor">The Journal of Urology .</a> <span>2016</span><span>,第2期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：关于无标记形态学评估精子细胞的干涉相显微镜</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_optical-methods-inspection-characterization-imaging-biomaterials-ii_thesis/0205135405.html">Influence of sample preparation and reliability of automated numerical refocusing in stain-free analysis of dissected tissues with quantitative phase digital holographic microscopy</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bjoern Kemper&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bjoern Kemper,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Philipp Lenz&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Philipp Lenz,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dominik Bettenworth&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Dominik Bettenworth,</a> <a href="/conference-foreign-1706/" target="_blank" rel="nofollow" class="tuijian_authcolor">Optical methods for inspection, characterization, and imaging of biomaterials II .</a> <span>2015</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：定量相数字全息显微镜对解剖组织的无污染分析中样品制备和自动数值重新聚焦的可靠性的影响</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061449117.html">Automated quantitative cellular analysis of immunohistochemically stained tissue sections.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Demirkaya, Omer.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Demirkaya, Omer. </a> <span>1997</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：免疫组织化学染色的组织切片的自动定量细胞分析。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040004324697.html">PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yair Rivenson&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yair Rivenson,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tairan Liu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Tairan Liu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhensong Wei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhensong Wei,</a> <span>2019</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：PhaseStain：使用深度学习对无标签定量相显微镜图像进行数字染色</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000129824978.html">Automated analysis of individual sperm cells using stain-free interferometric phase microscopy and machine learning</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Simcha K. Mirsky&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Simcha K. Mirsky,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Itay Barnea&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Itay Barnea,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mattan Levi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mattan Levi,</a> <span>2017</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用无染色干涉相位显微镜和机器学习自动分析单个精子细胞</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_information-recording-materials_thesis/0201271067488.html">三元阵相位干涉仪无模糊基线设计</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=肖香彬&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 肖香彬</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杜冶&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杜冶</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=任峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,任峰</a> <span> <a href="/journal-cn-9244/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 信息记录材料 </a> </span> <span> . 2019</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_systems-engineering-electronics_thesis/0201270301648.html">基于干涉相位的两步法高精度无模糊时延估计</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵培焱&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 赵培焱</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=彭华峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,彭华峰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=邓兵&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,邓兵</a> <span> <a href="/journal-cn-5216/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 系统工程与电子技术 </a> </span> <span> . 2018</span><span>,第011期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-national-university-defense-technology_thesis/020129016344.html">双天线干涉SAR系统无控制点场景的高精度参考相位快速估计算法</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈立福&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈立福</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=庞科臣&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,庞科臣</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李银伟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李银伟</a> <span> <a href="/journal-cn-7512/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 国防科技大学学报 </a> </span> <span> . 2017</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_systems-engineering-electronics_thesis/0201259113395.html">单通道干涉相位无模糊估计快速动目标速度</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张学攀&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张学攀</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=廖桂生&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,廖桂生</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=朱圣棋&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,朱圣棋</a> <span> <a href="/journal-cn-5216/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 系统工程与电子技术 </a> </span> <span> . 2014</span><span>,第009期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_measurement-technique_thesis/0201212745421.html">使用干涉显微镜的几点体会</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐云生&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 徐云生</a> <span> <a href="/journal-cn-7671/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 计量技术 </a> </span> <span> . 1991</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-57718_thesis/020222529580.html">光纤干涉仪的小相位检测——无衰落峰值抽检法</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈小宝&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈小宝</a> <span> <a href="/conference-cn-57718/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 全国第三次光纤通信学术会议 </a> <span> <span> . 1986</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020316259162.html">基于机器学习的电子散斑干涉相位提取新方法研究与应用</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈明明&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈明明</a> <span> . 2020</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061203686397.html">一种数字全息与微分干涉联合的双通道相移相位测量显微镜</a> <b>[P]</b> . <span> 中国专利： CN108303020B </span> <span> . 2020.04.03</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120110803257.html">一种数字全息与微分干涉联合的双通道相移相位测量显微镜</a> <b>[P]</b> . <span> 中国专利： CN108303020A </span> <span> . 2018-07-20</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130409433615.html">Methods to measure the total sperm concentration and sperm mobile in a sample and to determine the average speed of sperm cells and the percentage of sperm in a sample, system for measuring the concentration of total sperm in a sample and to examine the quality of semen. And, the sampling device for use in a biological fluid analysis optics</a> <b>[P]</b> . <span> 外国专利：  BRPI0117025B1 </span> <span> . 2016-12-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：测量样品中总精子浓度和可移动精子，确定精子细胞平均速度和样品中精子百分比的方法，测量样品中总精子浓度和检查精液质量的系统。并且，用于生物流体分析光学器件的采样装置 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130442512581.html">Methods to measure the total sperm concentration and sperm mobile in a sample and to determine the average speed of cells and sperm and the percentage of sperm in a sample.System for measuring the concentration of total sperm in a sample and to examine the quality of semen, and sampling system for use in a biological fluid analysis optics</a> <b>[P]</b> . <span> 外国专利：  BR0117025A </span> <span> . 2004-04-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：测量样品中总精子浓度和精子活动性的方法，确定细胞和精子的平均速度以及样品中精子百分比的方法测量样品中总精子浓度和检查精液质量的系统以及用于生物流体分析光学系统的采样系统 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130425355078.html">Methods for freezing sorted sperm cells comprising a protein containing sperm cell extender and a protein free cryoprotectant before freezing</a> <b>[P]</b> . <span> 外国专利：  NZ566129A </span> <span> . 2011-06-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：冷冻前冷冻分选的精子细胞的方法，所述精子细胞包括含有精子细胞增量剂的蛋白质和不含蛋白质的冷冻保护剂 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704022396893','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704022396893down" data-source="7," data-out-id="bzkVCI074ZY2uRpTHyvJ4Q==," data-f-source-id="7" data-title="Automated analysis of individual sperm cells using stain‐free interferometric phase microscopy and machine learning" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="17718" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Automated analysis of individual sperm cells using stain‐free interferometric phase microscopy and machine learning"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>