Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

Gilev K.V.; Yastrebova E.S.; Strokotov D.I.; Yurkin M.A.; Karmadonova N.A.; Chernyshev A.V.; Lomivorotov V.V.; Maltsev V.P.

摘要

Abstract >Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content. The above‐mentioned individual RBC characteristics were measured without fluorescent markers and other chemicals by a SFC equipped only with 660 nm laser for RBC illumination and single detector for measurement of angle‐resolved light scattering. The distributions over all RBC characteristics were constructed and processed statistically to form the novel 31 RBC indices for 22 donor samples. Our results confirm the possibility of precise, label‐free, enhanced morphological analysis of individual intact RBCs with compact single‐detector flow cytometer. Detailed characterization of RBCs with high statistics and precision can be used to increase the value of screening examinations and to reveal pathologies accompanied by abnormality of RBC shape. ? 2017 International Society for Advancement of Cytometry </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”> <title type =“main”>抽象</ title> >，而现代自动化血细胞分析仪测量音量单个红细胞（RBCS），导致四个RBC指数（平均碎石体积，MCV;平均碎石血红蛋白，MCH;平均碎石血红蛋白浓度，MCHC;红细胞分布宽度和RDW），RBC形状尚未评估通过临床筛选工具。我们施加了扫描流式细胞仪（SFC），以便在直径，最大和最小厚度，体积，表面积，球形指数，自发曲率，血红蛋白浓度和含量方面完全表征完整的RBC形态。通过仅配备660nm激光器的SFC用于RBC照明和单次检测器，测量上述单独的RBC特性，仅通过配备660nm激光器的SFC，用于测量角度分辨光散射。在统计上构建和处理所有RBC特性的分布，以形成22个供体样品的新型31 RBC索引。我们的结果证实了具有紧凑型单检测器流量仪的单个完整RBC的精确，无标记，增强的形态分析的可能性。详细表征具有高统计和精度的RBCs可用于增加筛选检查的价值，并揭示伴随RBC形状异常的病理学。还2017年国际促进细胞术社会</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-17718/'>《Cytometry, Part A: the journal of the International Society for Analytical Cytology》</a> <b style="margin: 0 2px;">|</b><span>2017年第9期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gilev K.V.&option=202" target="_blank" rel="nofollow">Gilev K.V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yastrebova E.S.&option=202" target="_blank" rel="nofollow">Yastrebova E.S.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Strokotov D.I.&option=202" target="_blank" rel="nofollow">Strokotov D.I.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yurkin M.A.&option=202" target="_blank" rel="nofollow">Yurkin M.A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Karmadonova N.A.&option=202" target="_blank" rel="nofollow">Karmadonova N.A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chernyshev A.V.&option=202" target="_blank" rel="nofollow">Chernyshev A.V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lomivorotov V.V.&option=202" target="_blank" rel="nofollow">Lomivorotov V.V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Maltsev V.P.&option=202" target="_blank" rel="nofollow">Maltsev V.P.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Voevodsky Institute of Chemical Kinetics and Combustion SB RAS Institutskaya 3Novosibirsk 630090 Russia;</p> <p>Voevodsky Institute of Chemical Kinetics and Combustion SB RAS Institutskaya 3Novosibirsk 630090 Russia;</p> <p>Voevodsky Institute of Chemical Kinetics and Combustion SB RAS Institutskaya 3Novosibirsk 630090 Russia;</p> <p>Voevodsky Institute of Chemical Kinetics and Combustion SB RAS Institutskaya 3Novosibirsk 630090 Russia;</p> <p>Siberian Biomedical Research Center Rechkunovskaya 15Novosibirsk 630055 Russia;</p> <p>Voevodsky Institute of Chemical Kinetics and Combustion SB RAS Institutskaya 3Novosibirsk 630090 Russia;</p> <p>Siberian Biomedical Research Center Rechkunovskaya 15Novosibirsk 630055 Russia;</p> <p>Voevodsky Institute of Chemical Kinetics and Combustion SB RAS Institutskaya 3Novosibirsk 630090 Russia;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/174.html" title="细胞生物学">细胞生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cell shape&option=203" rel="nofollow">cell shape;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=flow cytometry&option=203" rel="nofollow">flow cytometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=light scattering&option=203" rel="nofollow">light scattering;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=membrane curvature&option=203" rel="nofollow">membrane curvature;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=red blood cells&option=203" rel="nofollow">red blood cells;</a> </p> <div class="translation"> 机译：细胞形状;流式细胞术;光散射;膜曲率;红细胞; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022396891.html">Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gilev K.V.&option=202" target="_blank" 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href="/academic-journal-cn_journal-sichuan-university-medical-edition_thesis/0201296933417.html">无电流斑电击死兔的红细胞与血管内皮的扫描电镜观察</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王晔&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王晔</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘敏&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘敏</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李凡&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李凡</a> <span> <a href="/journal-cn-53738/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 四川大学学报：医学版 </a> </span> <span> . 2007</span><span>,第5期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-nanchang-university-medical-sciences_thesis/0201244722018.html">用流式细胞仪进行人核迁移蛋白shRNA有效干扰片段的分析和筛选</a> <b>[J]</b> <span> <a 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class="enjiyixqcontent" href="/patent-detail/06130402116584.html">Method of integrating bright field and fluorescence channels for cell image segmentation and morphological analysis using images acquired from an imaging flow cytometer (IFC)</a> <b>[P]</b> . <span> 外国专利：  JP6654271B2 </span> <span> . 2020-02-26</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用从成像流式细胞仪（IFC）获取的图像整合明场和荧光通道以进行细胞图像分割和形态分析的方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130404997168.html">Brightfield and fluorescence channel integration method for cell image segmentation and morphological analysis using images acquired from an imaging flow cytometer (IFC)</a> <b>[P]</b> . <span> 外国专利：  JP2019525199A </span> <span> . 2019-09-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用从成像流式细胞仪（IFC）获取的图像进行细胞图像分割和形态分析的明场和荧光通道整合方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130404165777.html">A Method To Combine Brightfield And Fluorescent Channels For Cell Image Segmentation And Morphological Analysis Using Images Obtained From Imaging Flow Cytometer (IFC)</a> <b>[P]</b> . <span> 外国专利：  US2019163956A1 </span> <span> . 2019-05-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：利用成像流式细胞仪（IFC）获得的图像，结合明场和荧光通道进行细胞图像分割和形态分析的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" 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