Luteimonas yindakuii sp. nov. isolated from the leaves of Dandelion (Taraxacum officinale) on the Qinghai-Tibetan Plateau

摘要

Two strains (S-1072(T) and 1626) of Gram-stain-negative, oxidase- and catalase-positive, aerobic, rod-shaped, motile bacteria with a single polar flagellum, were isolated from the leaves of Dandelion (Taraxacum officinale) on the Qinghai Tibet Plateau of China. The cells grew optimally at 28 degrees C, pH 7.0 and with 0.5% (w/v) NaCl on brain-heart infusion agar. Phylogenetic analysis based on 165 rRNA gene sequences revealed that strains S-1072(T) and 1626 belong to the genus Luteimonas, sharing the highest similarity with Luteimonas arsenica CCTCC AB 2014326(T) (97.0 %), Luteimonas terricola CGMCC 1.8985(T) (96.9 %) and Luteimonas aestuarii KCTC 22048(T) (96.6 %). The phylogenomic tree indicated that strains S-1072(T) and 1626 were most closely related to Luteimonas abyssi CGMCC 1.12611(T). The biochemical characteristics revealed that strains S-1072(T) and 1626 could neither produce trypsin nor produce acid from 0-glucose, N-acetylglucosamine and maltose, distinguishing them from four closest relatives. The DNA G+C contents of strains S-1072(T) and 1626 were 69.2 and 69.3 mol% respectively. The digital DNA-DNA hybridization values of our isolates with their four closely related species were below the 70% threshold. The predominant menaquinone was 0-8 (98.7 %) and the major polar lipids included diphosphatidylglycerol, phosphatidylglycerol and phos- phatidylethanolamine. The major fatty acids (>10%) were iso-C-15:0, iso-C(16:0 )and summed feature 9 (10-methyl C-16:0 and/or iso-C-17:1 omega 9c). Based on the data obtained, strains 5-1072(T) and 1626 should be classified as a novel species of the genus Luteimonas, for which the name Luteimonas yindakuii sp. nov. is proposed. The type strain is 5-1072(T) (=CGMCC 1.13927(T) =JCM 33487(T)).