Oocyte Development and Staging in the Florida Bass, Micropterus floridanus (LeSueur, 1822), with Comments on the Evolution of Pelagic and Demersal Eggs in Bony Fishes

摘要

Oocyte development and staging is described from histological preparation of the ovaries of field collected specimens of the Florida Bass, Micropterus floridanus. Oocyte development in Florida Bass progresses through six stages beginning with oogonial proliferation and ending with ovulation, the release of eggs. The stages are based on mitosis and meiosis, the core processes of Adaptable Oocyte Staging. After ovulation, the postovulatory follicle complex produces a mucuslike fluid with unknown function; we speculate that it protects the eggs immediately upon ovulation and upon their release into freshwater during spawning. Atresia is rare in the ovaries of reproductive Florida Bass. The release of cortical alveoli after fertilization follows a unique, extended time-release sequence. Oocyte development in the freshwater perciform Florida Bass follows precisely a pattern that has been documented for marine perciform fishes that produce positively buoyant, pelagic, non-adhesive eggs. The eggs of Florida Bass are morphologically like these pelagic eggs in that they have a single large oil globule and no attachment filaments. Yet Florida Bass eggs have small, clear yolk globules and an adhesive egg envelope and are functionally demersal. Thus, classification of an egg as pelagic or demersal should consider Its morphology as well as its function, especially if egg type is to be used to interpret phylogenetic relationships or evolutionary patterns. The clearing of yolk due to the uptake of water during the development of actinopterygian eggs, as they progress from a demersal to a potentially pelagic phase, may be a general mechanism of oocyte maturation in teleost fishes.