Ag-4-ATP-MWCNT electrode modified with dsDNA as label-free electrochemical sensor for the detection of daunorubicin anticancer drug

摘要

Abstract This paper describes the synthesis of Ag-4-ATP-MWCNT nanocomposite and its use as a modifier of working electrode. The surface of the electrochemical Ag-4-ATP-MWCNT electrode was modified with a double-stranded DNA (dsDNA) to detect daunorubicin-DNA interactions. Differential pulse voltammetry was applied to develop an electroanalytical procedure for the determination of daunorubicin and evaluate its interaction with dsDNA immobilized on the electrode surface. After the optimization of operational parameters, the linear dependence of the peak current on the daunorubicin concentration was observed in the range of 0.10×10?8 to 1.00×10?5 molL?1, with the detection and quantification limits of 3.00×10?10 and 1.00×10?9 molL?1, respectively. The proposed biosensor was successfully applied to validate its capability for the determination of daunorubicin in human serum and urine samples. Graphical abstract Display Omitted Highlights ? A DNA biosensor was developed for daunorubicin anticancer drug determination. ? Ag-4-ATP-MWCNT nanocomposite coated with dsDNA biosensor exhibits excellent sensitivity and selectivity. ? The as-prepared biosensor could be used for assay of serum and urine samples. ]]>