Generation and Identification ofMutations Resulting in Chronic andAge-Related Phenotypes in Mice

摘要

Aging is inevitable, and our society must deal with the consequences: namely,an increased incidence of disease and ill health. Many mouse models of diseaseare acute or early onset or are induced in young mice, despite the fact thataging is a significant risk factor for a range of significant diseases. To improvemodeling of such diseases, we should incorporate aging into our models. Manysystems are affected by aging, with a decline in mitochondrial function, anincrease in senescence, a loss of resilience, telomere shortening, and a declinein immune function being key factors in the increased susceptibility to diseasethat is associated with aging. To develop novel models of age-related disease,we undertook a phenotype-driven screen of a pipeline of mutagenized mice.Here, we describe some of the underlying protocols and outline importantaspects to consider when studying aged mice.