Pseudomonas jilinensis sp. nov., Isolated from Oil Production Water of Jilin Oilfield in China

摘要

A Gram-stain-negative, rod-shaped bacterial strain JS15-10A1(T) was isolated from oil production water. Its optimum growth was observed at 37 degrees C, pH 7.0, and 3% (w/v) NaCl. The 16S rRNA gene sequence of strain JS15-10A1(T) showed the highest similarities with Pseudomonas parafulva CB-1(T) (97.6%) and P. fulva IAM 1529(T) (97.5%). In addition, phylogenetic analyses based on multilocus sequence analyses with concatenating 16S rRNA, gyrB, rpoD, and rpoB genes indicated that strain JS15-10A1(T) was a member of genus Pseudomonas but discriminated from other species. Furthermore, whole-genome analyses revealed that average nucleotide identities and in silico DNA-DNA hybridization values of strain JS15-10A1(T) against its closest relatives were all below 76.7% and 21.1%, respectively. The major cellular fatty acids of strain JS15-10A1(T) were summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c), C-16:0, C-12:0, C-17:0 cyclo, summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c), C-12:0 3-OH, C-10:0 3-OH, and C-19:0 cyclo omega 8c. The predominant quinone was ubiquinone Q-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown amino-lipid, and two unidentified lipids. The genome DNA G + C content was 60.0 mol%. On the basis of phylogenetic, phenotypic, physiological, and chemotaxonomic analyses, it can be concluded that strain JS15-10A1(T) represents a novel species in genus Pseudomonas, for which the name Pseudomonas jilinensis sp. nov. is proposed. The type strain is JS15-10A1(T) (= CGMCC 1.16072(T) = LMG 30036(T)).