Fourier Transform Infrared Spectroscopy Applied to the Study of Unicellular Models

摘要

Background: Vibrational spectroscopy has shown the capacity to provide rapid, accurateand relevant information on microorganism’s classification and identification. In fact Fourier TransformInfrared Spectroscopy is a sensitive and effective methodology in food analysis, clinical diagnosisand environmental evaluation, with successful identification at genus and species level.Focus: The interest in this technique lies not only in the identification and classification of bacteria,but there is also relevance in using FTIR to follow cellular responses to stress in unicellular models,with particular interest in yeasts and so far results show FTIR is able to evaluate specific cellularchanges related to stress stimulus, mainly in protein region.Prospect: This work gathered information that demonstrates that it is possible to overcome signal overlappingand use Fourier Transform Infrared Spectroscopy and multivariate analysis tools to obtain biochemicalsignatures of microorganisms and corresponding metabolome that can provide inexpensiveand highly specific alternatives to conventional typing methods. Also, it presents a spectrum representativeof a microbial cell and summarizes in a table the spectral assignments of main biological contributors.