Identification of known leaf rust resistance genes in common wheat cultivars from Sichuan province in China

摘要

Leaf rust, caused byPuccinia triticina(Pt), is one of the most important wheat diseases of common wheat (Triticum aestivumL.). Using resistant cultivars is the most economical and efficient way to control the disease. A total of 86 wheat cultivars from Sichuan province in China were inoculated with 14?Ptraces for postulating seedling leaf rust resistance (Lr) gene(s) in the greenhouse. Meanwhile, these cultivars were also planted in Baoding and Zhoukou trial fields for identifying slow leaf rusting genes during the 2014–2015 and 2015–2016 cropping seasons. Twelve specific markers for ten knownLrgenes (Lr1,Lr9,Lr10,Lr19,Lr20,Lr24,Lr26,Lr34,Lr37andLr46) were also used for molecular marker detection. Based on the results from the gene postulation and molecular marker detection, nineLrgenes (Lr1,Lr2a, Lr26,Lr3ka,Lr30,Lr36,Lr15,Lr37, andLr46) were identified in 45 cultivars either singly or in combination. Most frequently identifiedLrgenes wereLr26in 25 cultivars andLr37in 21 cultivars. Less frequently detected genes wereLr1andLr46each in nine cultivars,Lr3kain five cultivars,Lr30in three cultivars andLr36in two cultivars.Lr2aandLr15were found in Chuannong 16 and Chuanmai 1, respectively. Twenty-nine cultivars were found to have slow rusting resistance during the two growing seasons. The results should be useful for selecting cultivars with combinations of genes for effective resistance to grow and in breeding new cultivars with improved resistance to leaf rust.