P-28 Experimental study of cleft lip and palate (No. 130) cleft palate after palatal fusion in mice exposed to TCDD

摘要

Introduction: We had reported that the mice embryos exposed TCDD, the minimum concentration to induce a 100% cleft palate rate, occurred cleft palates after palatal fusion. We studied to reveal the involvement of intercellular adhesion in the cleft palates after palatal fusion. Methods: In this study, on GD 12, ICR pregnant mice were administered with TCDD diluted in olive oil at a dose of 40 μg/ kg body weight, the minimum concentration to induce a 100% cleft palate rate (TCDD-exposed embryos). The palates with fusion of TCDD-exposed embryos compared with the palates of control embryos in immunohistochemistry. Results: The appearance of intercellular adhesion factor was different at the fusional palate between control embryos and TCDD-exposed embryos. Discussion: From this study, we suggested that the intercellular adhesion concerned the epithelial split and the mesenchymal defect of the fusional palate, and there was a possibility that the weakness of intercellular adhesion led to the cleft palate after palatal fusion.