Combining VITEK (R) 2 with colistin agar dilution screening assist timely reporting of colistin susceptibility

摘要

Objectives: The rise in carbapenem resistance among Gram-negative bacteria has renewed interest in colistin. Recently, the EUCAST-CLSI Polymyxin Breakpoints Working Group declared that broth microdilution (BMD) is the only valid method for colistin susceptibility testing. BMD is not easily incorporated into the routine work of clinical laboratories, and usually this test is incorporated serially, resulting in delayed susceptibility reporting. We tested a strategy of combining VITEK (R) 2 with a 2 mu g/mL colistin agar dilution (VITEK (R) 2/AD) screening plate to improve performance and time to reporting of colistin susceptibility.