Detection of Toxoplasma gondii in infected meat samples by a latex agglutination test using anti-P30 recombinant antibody

摘要

Detection of Toxoplasma gondii in meat products, which is responsible for severe disease in humans and in animals, is a great challenge. Recombinant antibodies (rAbs) have been offered as valuable tools for different diagnostic and therapeutic goals. In this study, a latex agglutination assessment for identification of Toxoplasma gondii in contaminated meat products was developed. For this propose, recombinant anti-P30 single-chain Fv (scFv) antibody gene was cloned into pET26b vector to express this antibody in high level. This gene's expression in Escherichia coli lead to production of significant amount of antibody (16 mg/L). After purification, the anti-P30 scFv was connected to beads of latex and used for agglutination test. These beads could agglutinate whole Toxoplasma gondii and contaminated meat samples. Thus, this rapid test may be applied to find contaminated samples.