Development of molecular markers linked to several fruit traits in oriental pear.

摘要

To develop molecular markers linked to fruit traits, several important fruit characteristics such as skin colour, the amount of grit cells, hardness, core size and weight were assessed and genetically analysed in 135 F1 pear seedlings from the cross between Pyrus pyrifolia cv. Niitaka and P. ussuriensis cv. Suhyangri. All the studied characteristics, except the grit cell content, showed a normal distribution. To detect molecular markers linked to several fruit characteristics, high quality genomic DNAs were extracted and sorted into two DNA pools by bulked segregant analysis. Two RAPD and 7 AFLP markers were selected. OPD05-850 and OPD08-500, linked to fruit hardness and skin colour respectively, were selected by RAPD analysis. Eight AFLP markers out of the 64 primer combinations were selected (EaaMcag-440 and EaggMcag-470 linked to skin colour; EacaMcac-259, EacaMctt-330 and EagcMcta-170 linked to grit cell content; EaacMctg-350 linked to fruit hardness; and EacaMctt-340 and EaagMctg-330 linked to fruit shape). The selected markers were cloned and sequenced for use as stable co-dominant markers for the increase of pear breeding efficiency. The 2 AFLP markers linked with the amount of grit cells were sequenced successfully from EacaMcac-259 and EacaMctt-330 with their original marker sequences..