Progress in structuring and maintaining the garlic (Allium sativum) diversity for the European GENRES project.

摘要

A part of the Gatersleben garlic (Allium sativum) collection, amounting to 50 accessions has been selected for morphological characterization and establishment of virus-free in vitro clones. Several characters have been described which more or lessclosely correspond to previously found isoenzyme/random amplified polymorphic DNA groups. The characters describing the inflorescence formation, bulb structures and pseudostem shape have been analysed. The clones have been screened for virus infection bymeans of 6 different ELISA tests. The infection rates varied from 8 mite-borne filaments virus [garlic mite-borne filamentous virus] (GarMbFV) to 94% garlic common latent virus (GCLV). Most accessions were infected with more than one virus, mostly latent viruses accompanying another form. Meristem cultures were performed to eliminate the viruses. Meristem explants reacted in 8-33%. The regenerated plantlets have been retested by ELISA. The results showed that a total of 38% of the plantlets have been proved virus-free.