Lysine-Appended Polydiacetylene Scaffolds for Human Mesenchymal Stem Cells

摘要

We report on the self-assembly based fabrication of fibrous, ppiymers for tissue engineering applications. Directed self-assembly followed by: polymerization of lysine-appended diacetylehes. generated a variety of polymers (Pi— PS) with distinct chemical properties. The self-assembly along with the conjugated double, and triple bonds and rigid geometry of diacetylehe; backbone imposed a nariofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys N~α-NH2 and Lys N~ε-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with N~ε-Boc and N~α-Fmoc or more hydrophilic polymer PS containing free N~ε and N~α amino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.