Molecular markers in detection of distant hybrids in Lilium.

摘要

Plants were obtained by ovule rescue from pollinations of lily cultivars belonging to the group "oriental hybrids" with pollen of Lilium henryi, L. pumilum and L. formolongi. To develop molecular DNA markers useful for separation of the hybrids allthe parental genotypes, resulting seedlings and regenerants were fingerprinted using RAPDs. The products of amplification were detected for all the forty primers used. Eight primers generated reproducible polymorphic bands within the pair 'Marco Polo' and L. henryi, six primers within 'Alma Ata' and L. pumilum, and 'Muscadet' and L. formolongi. It was found that some bands could serve as markers to detect hybrids at the in vitro stage.