Isolation of tissue culture-induced polymorphisms in bananas by representational difference analysis.

摘要

Representational difference analysis is a comparison between two DNAs by using one of them in excess in a hybridization reaction to remove all the sequences held in common between the two DNAs. Therefore, what remains are those sequences which differ between the two. This technique was applied to DNAs extracted from normal and off-types of banana as well as to plants of different genomic constitutions. The results showed that this proved a useful technique for identifying polymorphisms arising during the process of tissue culture. Both of the different clones characterized appeared to be the result of a genomic arrangement rather than a change in methylation status.